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IMMUNIS HBV Genotype EIA

Institute of Immunology,Co.,Ltd.

Institute of Immunology,Co.,Ltd.

URL : http://www.tokumen.co.jp/english/

EIA kit for serological determination of HBV genotype

IMMUNIS HBV Genotype EIA

There are eight genotypes (genotype A to H) of hepatitis B virus (HBV) which are distinguished by > 8 % difference in the nucleotide sequence of the HBV genome 1, 2). Genotypes A and D are common in Europe and the United States, while genotypes B and C are prevalent in Asia. Genotype F is frequent in Latin America, while genotype E is confined to Central and Western Africa.

Many studies indicate that HBV genotypes influence the severity of chronic hepatitis B and the response to interferon therapy. HBV genotypes have been determined by tedious methods such as direct sequencing, genotype-specific PCR, PCR-RFLP, line-probe assay (LiPA), or PCR-invader assay.

This product is an enzyme immunoassay (EIA) based HBV genotyping kit, designed to determine genotype (A, B, C, or D) by detecting genotype-specific epitopes in PreS2 region 3-5).

pdfInstruction Manual(403KB)

Note: This product is intended to be used only for research purpose outside Japan.


Application
Hepatitis B Virus serological genotyping in human serum (Genotype A, B, C, or D)
Features
  • ● The first diagnostic reagent for determination of HBV genotype launched in the Japanese market
  • ● Fast and easy detection based on one-step EIA method
  • ● 98% of samples with HBsAg concentration of >3 IU/mL can be genotyped (unpublished data)
  • ● Sample volume: 80μL of HBsAg positive serum
  • ● Stored sample can be used
  • ● 4 wells/sample
  • ● Reaction time: 2.5 hours
Product Name
IMMUNIS® HBV Genotype EIA
Product Code
1A65
Package
48 tests
References
  1. Okamoto H, Tsuda F, Sakugawa H, et al:Typing hepatitis B virus by homology in nucleotide sequence:comparison of surface antigen subtypes. J Gen Virol 69:2575-2583, 1988.
  2. Arauz-Ruiz P, Norder H, Robertson BH, et al:Genotype H:a new Amerindian genotype of hepatitis B virus revealed in Central America. J Gen Virol 83:2059-2073, 2002.
  3. Usuda S, Okamoto H, et al: Serological detection of hepatitis B virus genotypes by ELISA with monoclonal antibodies to type-specific epitopes in the preS2-region product. J Virol Methods 80:97-112, 1999.
  4. Moriya T, Kuramoto IK, Yoshizawa H, et al: Distribution of hepatitis B virus genotypes among American blood donors determined with a PreS2 epitope enzyme-linked immunosorbent assay kit. J Clin Microbiol. 40: 877-880, 2002.
  5. Tanaka Y, Sugauchi F, Matsuura K, et al: Evaluation of hepatitis B virus genotyping EIA kit. Rinsho Byori. 57: 42-47, 2009.
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