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Human renal cell line ciPTEC – Radboud University

ciPTEC (conditionally immortalized Renal Proximal Tubule Epithelial Cell) is a novel cell line developed by Radboud University. ciPTEC is conditionally immortalized by using SV40 large T antigen and human telomerase reverse transcriptase (hTERT). ciPTEC differs from existing cell lines because it stably expresses most transporters.

ciPTEC: stable expression of relevant renal drug transporters

ABCB1 = P-glycoprotein/MDR1
ABCC2 = multidrug resistance protein 2 (MRP2)
ABCC4 = multidrug resistance protein 4 (MRP4)
ABCG2 = breast cancer resistance protein (BCRP)
SLC22A2 = organic cation transporters (OCT2)
SLC22A6 = organic anion transporters (OAT1)
SLC22A8 = organic anion transporters (OAT3)
SLC47A1 = multidrug and toxin extrusion transporter (MATE1)
SLC47A2 = multidrug and toxin extrusion transporter (MATE2-k)
SLCO4C1 = organic anion transporting polypeptide (OATP-H)


Reliable prediction of drug-induced kidney injury (DIKI) is a major issue during drug development. 1 out of 3 lead compounds lead to organ toxicity and 1 out of 5 compounds lead to renal toxicity during phase 3 of drug development. ciPTEC expresses all relevant drug transporters. This cell line provides a robust and predictive model for ADME/tox studies during drug development.

ciPTEC has the potential to reduce animal experiments in the future as highly predictive in vitro renal toxicity model

Features of ciPTEC

● Human origin
● Endogenous and stable expression of most renal transporters
● Stably introduced OAT1 and OAT3
● Functional assays for DDI and toxicity
● Stable for > 60 passage numbers
● > 30 scientific publications

Applications for ciPTEC

● Drug-drug interaction studies
● Influx assays (OCT2, OAT1/3)
● Efflux assays (Pgp, BCRP, MRP4)
● Cell toxicity
● Phosphate reabsorption
● Receptor mediated endocytosis


Cell line list

Name Description
ciPTEC-parent conditionally immortalized human proximal tubule cell line stably expressing a range of drug transporters
ciPTEC-OAT1 conditionally immortalized human proximal tubule cell line stably expressing a range of drug transporters, including stable expression of OAT1

※Please inquire us for licensing of these cell lines.


References

  1. Wilmer MJ et al., Novel conditionally immortalized human proximal tubule cell line expressing functional influx and efflux transporters. Cell Tissue Res 339: 449-457, 2010
  2. Schophuizen CMS et al. Cationic uremic toxins affect renal proximal tubule cell functioning through interaction with the organic cation transporter (hOCT2). Pflügers Archives 465(12):1701-14, 2013
  3. Jansen J et al. A morphological and functional comparison of proximal tubule cell lines established from human urine and kidney tissue. Exp Cell Res. 323(1):87-99, 2014
  4. Peters E et al. Alkaline phosphatase protects against renal inflammation through dephosphorylation of lipopolysaccharide and adenosine triphosphate. Br J Pharmacol. 172(20):4932-45; 2015
  5. Nieskens TT et al. A Human Renal Proximal Tubule Cell Line with Stable Organic Anion Transporter 1 and 3 Expression Predictive for Antiviral-Induced Toxicity. AAPS J. 18(2):465-75, 2016
  6. Caetano-Pinto P. et al. Fluorescence based transport assays revisited in a human renal proximal tubule cell line. Mol Pharm. 2016, 2016 Mar 7;13(3):933-44


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