Radboud University – Human renal cell line ciPTEC

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ciPTEC (conditionally immortalized Renal Proximal Tubule Epithelial Cell) is a novel cell line developed by Radboud University. ciPTEC is conditionally immortalized by using SV40 large T antigen and human telomerase reverse transcriptase (hTERT). ciPTEC differs from existing cell lines because it stably expresses most transporters.

 
ciPTEC: stable expression of relevant renal drug transporters

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ABCB1 = P-glycoprotein/MDR1
ABCC2 = multidrug resistance protein 2 (MRP2)
ABCC4 = multidrug resistance protein 4 (MRP4)
ABCG2 = breast cancer resistance protein (BCRP)
SLC22A2 = organic cation transporters (OCT2)
SLC22A6 = organic anion transporters (OAT1)
SLC22A8 = organic anion transporters (OAT3)
SLC47A1 = multidrug and toxin extrusion transporter (MATE1)
SLC47A2 = multidrug and toxin extrusion transporter (MATE2-k)
SLCO4C1 = organic anion transporting polypeptide (OATP-H)

 

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Reliable prediction of drug-induced kidney injury (DIKI) is a major issue during drug development. 1 out of 3 lead compounds lead to organ toxicity and 1 out of 5 compounds lead to renal toxicity during phase 3 of drug development. ciPTEC expresses all relevant drug transporters. This cell line provides a robust and predictive model for ADME/tox studies during drug development.

ciPTEC has the potential to reduce animal experiments in the future as highly predictive in vitro renal toxicity model

 

 
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Features of ciPTECの特徴

● Human origin
● Endogenous and stable expression of most renal transporters
● Stably introduced OAT1 and OAT3
● Functional assays for DDI and toxicity
● Stable for > 60 passage numbers
● > 30 scientific publications
 

 

Applications of ciPTEC

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● Drug-drug interaction studies
● Influx assays (OCT2, OAT1/3)
● Efflux assays (Pgp, BCRP, MRP4)
● Cell toxicity
● Phosphate reabsorption
● Receptor mediated endocytosis
 
 
 


 

Cell line list 

Name Description
ciPTEC-parent conditionally immortalized human proximal tubule cell line stably expressing a range of drug transporters
ciPTEC-OAT1 conditionally immortalized human proximal tubule cell line stably expressing a range of drug transporters, including stable expression of OAT1

※Please inquire us for licensing of these cell lines.

 


 

References

 
  • Wilmer MJ et al., Novel conditionally immortalized human proximal tubule cell line expressing functional influx and efflux transporters. Cell Tissue Res 339: 449-457, 2010
     
  • Schophuizen CMS et al. Cationic uremic toxins affect renal proximal tubule cell functioning through interaction with the organic cation transporter (hOCT2). Pflügers Archives 465(12):1701-14, 2013
     
  • Jansen J et al. A morphological and functional comparison of proximal tubule cell lines established from human urine and kidney tissue. Exp Cell Res. 323(1):87-99, 2014
     
  • Peters E et al. Alkaline phosphatase protects against renal inflammation through dephosphorylation of lipopolysaccharide and adenosine triphosphate. Br J Pharmacol. 172(20):4932-45; 2015
     
  • Nieskens TT et al. A Human Renal Proximal Tubule Cell Line with Stable Organic Anion Transporter 1 and 3 Expression Predictive for Antiviral-Induced Toxicity. AAPS J. 18(2):465-75, 2016
     
  • Caetano-Pinto P. et al. Fluorescence based transport assays revisited in a human renal proximal tubule cell line. Mol Pharm. 2016, 2016 Mar 7;13(3):933-44

 

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